Detection method details
Name: |
QL-EVE-TA-MON71200 |
Description: |
Qualitative real-time PCR (TaqMan) method for detection of wheat event MON71200 (EU-RL GMFF GMOMETHODS database as of July 3, 2018). |
Comment: |
Target is the 3' integration border region (IBR) between the insert of wheat event MON71200 and the wheat host genome.
Event-specific diagnostic method for the detection of MON71200 wheat (Simplex). Developed by the Genotyping – Botany Laboratory, Ottawa Plant Laboratory, Canadian Food Inspection Agency CFIA). |
Validation: |
in-house validation |
Standardisation: |
EU reference method |
Related Methods: |
Type: |
event-specific |
Target GMO name: |
MON71200 |
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Oligonucleotides:
Forward Primer
Name:
MON71200-3 ’ junction-1FSequence:
CACGACGGTCATCGAGCSize:
17
Reverse Primer
Name:
MON71200-3 ’ junction-1RSequence:
CCGTTCGTCATTGACTGTTSize:
19
Probe
Name:
MON71200-3 ’ junction-PSequence:
CATACGGAAAAGATGCTGCAGGGAATATATTGAACSize:
35
-
Amplicon:
Sequence:
Size:
139
Documents
Citation | Type | Local copy |
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