Detection method details
Name: |
QL-EVE-375LibF/376LibR |
Description: |
Qualitative real-time PCR (TaqMan) method for detection of oilseed rape event Liberator (pHoe6/Ac) (BVL G30.40-6). |
Comment: |
Target is the integration border region (IBR) between the insert of oilseed rape event Liberator (pHoe6/Ac) and the rapeseed host genome. |
Validation: |
ring trial validation |
Standardisation: |
national standard |
Related Methods: |
QT-TAX-BN-012 |
Type: |
event-specific |
Target GMO name: |
Liberator |
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Oligonucleotides:
Forward Primer
Name:
375LibFSequence:
CCATATTGACCATCATACTCCCCSize:
23
Reverse Primer
Name:
376LibRSequence:
TGACTTTGAACAATAATTTCAGTAACATATASize:
28
Probe
Name:
377LibSSequence:
CCTGATGTAAACAATAAAATTSize:
21
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Amplicon:
Sequence:
ccatattgaccatcatactccccccctgatgtaaacaataaaattggtataattggagaatttactatatgttactgaaattattgttcaaagtcaSize:
96