Detection method details
Name: |
QT-TAX-ZM-011 |
Description: |
Quantitative real-time PCR (TaqMan) method for detection of the maize alcohol dehydrogenase 1 gene (EU-RL GMFF GMOMETHODS database as of March 06, 2014) |
Comment: |
Target is the endogenous alcohol dehydrogenase 1 (adh1) gene from Zea mays
ATTENTION: Following Broothaerts et al. (2008: J Agric Food Chem, 56:8825-8831) a common allelic single nucleotide polymorphism (SNP) within the nucleotide sequence of adh1 of maize at position 839 relative to the translational start codon of the gene may hinder the efficient binding of the reverse primer Adh1 primer R. Therefore, quantification of genetically modified maize events on the basis of this method might be impaired. |
Validation: |
ring trial validation |
Standardisation: |
EU reference method |
Related Methods: |
Type: |
taxon-specific |
Target Species name: |
Zea mays |
-
Oligonucleotides:
Forward Primer
Name:
Adh1 primer FSequence:
CCAGCCTCATGGCCAAAGSize:
18
Reverse Primer
Name:
Adh1 primer RSequence:
CCTTCTTGGCGGCTTATCTGSize:
20
Probe
Name:
Adh1 probeSequence:
CTTAGGGGCAGACTCCCGTGTTCCCTSize:
26
-
Amplicon:
Sequence:
ccttcttggcggcttatctgncttaggggcagactcccgtgttccctnnnnnctttggccatgaggctggSize:
70
Documents
Citation | Type | Local copy |
---|---|---|
EU-RL GMFF GMOMETHODS database; QT-TAX-ZM-011 | EU reference method protocol |