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Detection Image Detection method details

Name:

 QT-TAX-ZM-006

Description:

 Quantitative real-time PCR (TaqMan) method for detection of maize starch synthase IIb gene (ISO 21570)

Comment:

 Target is the endogenous starch synthase IIb (zSSIIb) gene from Zea mays

Validation:

 ring trial validation

Standardisation:

 ISO standard

Related Methods:

Type:

 taxon-specific

Target Species name:

 Zea mays

  • Oligonucleotides:

  • Forward Primer

  • Name:

    SSllb 1-5'

  • Sequence:

    CTCCCAATCCTTTGACATCTGC

  • Size:

    22

  • Reverse Primer

  • Name:

    SSIIb 1-3'

  • Sequence:

    TCGATTTCTCTCTTGGTGACAGG

  • Size:

    23

  • Probe

  • Name:

    SSIIb-Taq

  • Sequence:

    AGCAAAGTCAGAGCGCTGCAATGCA

  • Size:

    25

  • Amplicon:

  • Sequence:

    ctcccaatcctttgacatctgcnnnnnagcaaagtcagagcgctgcaatgcannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncctgtcaccaag agagaaatcga

  • Size:

    151

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-TAX-ZM-006Link to the document url. EU reference method protocol
Shindo Y., Kuribara H., Matsuoka T., Futo S., Sawada C., Shono J., Akiyama H., Goda Y., Toyoda M., Hino A.; Validation of real-time PCR analyses for line-specific quantitation of genetically modified maize and soybean using new reference molecules" J AOALink to the document url.