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Detection Image Detection method details

Name:

 QT-EVE-ZM-035

Description:

 Quantitative real-time PCR (TaqMan) method for detection of maize event DP23211 (EU-RL GMFF GMOMETHODS database as of February 15, 2024)

Comment:

 Target is the 3' integration border region (IBR) between the insert of maize event DP23211 and the maize host genome

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

Type:

 event-specific

Target GMO name:

 DP23211

  • Oligonucleotides:

  • Forward Primer

  • Name:

    PHN175787_f

  • Sequence:

    TTACGGCATCTAGGACCGACTAG

  • Size:

    23

  • Reverse Primer

  • Name:

    PHN175788_r

  • Sequence:

    GAAGCACTTGTTTTTCAATTCCAA

  • Size:

    24

  • Probe

  • Name:

    PHN175789_p

  • Sequence:

    CTAGTACGTAGTGAATCTG

  • Size:

    19

  • Amplicon:

  • Sequence:

    ttacggcatctaggaccgactagnnnnnnnnctagtacgtagtgaatctgnttggaattgaaaaacaagtgcttc

  • Size:

    75

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-EVE-ZM-035Link to the document url. EU reference method protocol