Help icon

Detection Image Detection method details

Name:

 QL-ELE-P-TA29-F/P-TA29-R

Description:

 Qualitative real-time PCR (TaqMan) method for detection of P-TA29 tapetal specific promoter of Nicotiana tabacum (Debode et al., 2013)

Comment:

 Target is the P-TA29 tapetal specific promoter (P-TA29) from Nicotiana tabacum

Validation:

 in-house validation

Standardisation:

 none

Related Methods:

Type:

 element-specific

Target DNA element:
P-ta29-TOBAC

  • Oligonucleotides:

  • Forward Primer

  • Name:

    P-TA29-F

  • Sequence:

    GAAGCTGTGCTAGAGAAGATGTTTATTC

  • Size:

    28

  • Reverse Primer

  • Name:

    P-TA29-R

  • Sequence:

    GCTCGAAGTATGCACATTTAGCAA

  • Size:

    24

  • Probe

  • Name:

    P-TA29-P

  • Sequence:

    AGTCCAGCCACCCACCTTATGCAAGTC

  • Size:

    27

  • Amplicon:

  • Sequence:

    GAAGCTGTGCTAGAGAAGATGTTTATTCTAGTCCAGCCACCCACCTTATGCAAGTCTGCTTTTAGCTTGATTCAAAAACTGATTTAATTTACATTGCTAAATGTGCATACTTCGAGC

  • Size:

    117

Documents

Citation Type Local copy
Debode, F., Janssen, E., Berben, G. (2013). Development of 10 new screening PCR assays for GMO detection targeting promoters (pFMV, pNOS, pSSuAra, pTA29, pUbi, pRice actin) and terminators (t35S, tE9, tOCS, tg7). Eur Food Res Technol, 236, 659–669.Link to the document url. peer-reviewed article 17-09-2015