Detection method details
Name: |
QL-ELE-Patf-n2/Patr-n2 |
Description: |
Qualitative real-time PCR (TaqMan) method for detection of the pat gene (Xu et al., 2006) |
Comment: |
target is the phosphinothricin N-acetyltransferase (pat) gene from bacterium Streptomyces viridochromogenes |
Validation: |
in-house validation |
Standardisation: |
none |
Related Methods: |
Type: |
element-specific |
Target DNA element: |
CS-pat-STRVR
|
-
Oligonucleotides:
Forward Primer
Name:
Patf-n2Sequence:
GACAGAGCCACAAACACCACAASize:
22
Reverse Primer
Name:
Patr-n2Sequence:
CAATCGTAAGCGTTCCTAGCCTSize:
22
Probe
Name:
Patp-n2Sequence:
GCCACAACACCCTCAACCTCASize:
21
-
Amplicon:
Sequence:
GACAGAGCCACAAACACCACAAGAGTGGATTGATGATCTAGAGAGGTTGCAAGATAGATACCCTTGGTTGGTTGCTGAGGTTGAGGGTGTTGTGGCTGGTATTGCTTACGCTGGGCCCTGGAAGGCTAGGAACGCTTACGATTGSize:
144