Detection method details
Name: |
QT-EVE-GH-008 |
Description: |
Quantitative real-time PCR (TaqMan) method for detection of cotton event GHB119 (EU-RL GMFF GMOMETHODS database as of July 21, 2014). |
Comment: |
Target is the 3' integration border region (IBR) between the insert of cotton event GHB119 and the cotton host genome. |
Validation: |
ring trial validation |
Standardisation: |
EU reference method |
Related Methods: |
Type: |
event-specific |
Target GMO name: |
GHB119 |
-
Oligonucleotides:
Forward Primer
Name:
SHA021Sequence:
CCAGTACTAAAATCCAGATCATGCASize:
25
Reverse Primer
Name:
NEL109Sequence:
GAAATTGCGTGACTCAAATTCCSize:
22
Probe
Name:
TM082Sequence:
CCTGCAGGTCGACGGCCGAGTACSize:
23
-
Amplicon:
Sequence:
ccagtactaaaatccagatcatgcannnncctgcaggtcgacggccgagtacnnnnnnnnnnnnnnnnggaatttgagtcacgcaatttcSize:
90
Documents
Citation | Type | Local copy |
---|---|---|
EU-RL GMFF GMOMETHODS database; QT-EVE-GH-008 | EU reference method protocol |