Detection method details
Name: |
QT-EVE-ZM-004 |
Description: |
Quantitative real-time PCR (TaqMan) method for maize event DAS40278 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) |
Comment: |
Target is the 5' integration border region (IBR) between the insert of maize event DAS40278 and the maize host genome |
Validation: |
ring trial validation |
Standardisation: |
EU reference method |
Related Methods: |
QT-TAX-ZM-002 |
Type: |
event-specific |
Target GMO name: |
DAS40278 |
-
Oligonucleotides:
Forward Primer
Name:
DAS-40278-9_5'-f1Sequence:
CACGAACCATTGAGTTACAATCSize:
22
Reverse Primer
Name:
DAS-40278-9_5'-r3Sequence:
TGGTTCATTGTATTCTGGCTTTGSize:
23
Probe
Name:
DAS-40278-9_5'-S2Sequence:
CGTAGCTAACCTTCATTGTATTCCGSize:
25
-
Amplicon:
Sequence:
cacgaaccattgagttacaatcnnnnnnnnnnnnnnnnnnnncggaatacaatgaaggttagctacgnnnnnnnncaaagccagaatacaatgaaccaSize:
98
Documents
Citation | Type | Local copy |
---|---|---|
EU-RL GMFF GMOMETHODS database; QT-EVE-ZM-004 | EU reference method protocol |