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Detection Image Detection method details

Name:

 QL-ELE-00-026

Description:

 Qualitative real-time PCR (TaqMan) method for detection of the element coding for phosphinothricin N-acetyltransferase (CS-bar-STRHY) gene from Streptomyces hygroscopicus (partim bar) (Debode et al., 2016).

Comment:

 Target is the phosphinothricin N-acetyltransferase (CS-bar-STRHY) gene from the bacterium Streptomyces hygroscopicus

For details of the detection of pat (partim pat) by this duplex real-time PCR method refer to QL-ELE-00-025

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

Type:

 element-specific

Target DNA element:
CS-bar-STRHY

  • Oligonucleotides:

  • Forward Primer

  • Name:

    RapB-F1

  • Sequence:

    acaagcacggtcaacttcc

  • Size:

    19

  • Reverse Primer

  • Name:

    RapB-R1

  • Sequence:

    gaggtcgtccgtccactc

  • Size:

    18

  • Probe

  • Name:

    RapB-S1

  • Sequence:

    taccgagccgcaggaacc

  • Size:

    18

  • Amplicon:

  • Sequence:

    acaagcacggtcaacttccgtaccgagccgcaggaaccgcaggagtggacggacgacctc

  • Size:

    60

Documents

Citation Type Local copy
Debode F., Huber I., Macarthur R., et al. (2016) Inter-laboratory studies for the validation of two singleplex (tE9 and pea lectin) and one duplex (pat/bar) real-time PCR methods for GMO detection. Food Control, 73, 452-461Link to the document url. peer-reviewed article
EU-RL GMFF GMOMETHODS database; QL-ELE-00-026Link to the document url. EU reference method protocol