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Detection Image Detection method details

Name:

 QL-ELE-00-005

Description:

 Qualitative conventional PCR method for detection of Cauliflower mosaic virus 35S promoter (ISO/FDIS 21569)

Comment:

 Target is the Cauliflower mosaic virus 35S promoter (CaMV P-35S)

Validation:

 ring trial validation

Standardisation:

 ISO standard

Related Methods:

Type:

 element-specific

Target DNA element:
P-Cauliflower mosaic virus

  • Oligonucleotides:

  • Forward Primer

  • Name:

    35S-1

  • Sequence:

    GCTCCTACAAATGCCATCA

  • Size:

    19

  • Reverse Primer

  • Name:

    35S-2

  • Sequence:

    GATAGTGGGATTGTGCGTCA

  • Size:

    20

  • Amplicon:

  • Sequence:

    gctcctacaaatgccatcannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntgacgcacaatcccactatc

  • Size:

    195

Documents

Citation Type Local copy
Foodstuffs - Methods of analysis for the detection of genetically modified organisms and derived products - Qualitative nucleic acid based methods ISO 21569:1-69 (2005)Link to the document url. other document
EU-RL GMFF GMOMETHODS database; QL-ELE-00-005Link to the document url. EU reference method protocol