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Detection Image Detection method details

Name:

 QT-EVE-GH-013

Description:

 Quantitative real-time PCR (TaqMan) method for detection of cotton event GHB811 (EU-RL GMFF GMOMETHODS database as of August 5, 2020)

Comment:

 Target is the 5' integration border region (IBR) between the insert of cotton event GHB811 and the cotton host genome

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

 QT-TAX-GH-020

Type:

 event-specific

Target GMO name:

 GHB811

  • Oligonucleotides:

  • Forward Primer

  • Name:

    PRIM0638

  • Sequence:

    CGAATAGTTCCATCAATTTTATCATTTATG

  • Size:

    30

  • Reverse Primer

  • Name:

    PRIM1870

  • Sequence:

    CGCTTTAACGTCCCTCAGATTT

  • Size:

    22

  • Probe

  • Name:

    TM2207

  • Sequence:

    AAGCCTTGAAACAGAACA

  • Size:

    18

  • Amplicon:

  • Sequence:

    cgaatagttccatcaattttatcatttatgnnnnnnnaagccttgaaacagaacannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnaaatctgagggacgttaaagcg

  • Size:

    144

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-EVE-GH-013Link to the document url. EU reference method protocol