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Detection Image Detection method details

Name:

 QL-ELE-bar 2-5'/bar 2-3'

Description:

 Qualitative real-time PCR (TaqMan) method for detection of the bar gene (Mano et al., 2009)

Comment:

 target is the phosphinothricin N-acetyltransferase (bar) gene from bacterium Streptomyces hygroscopicus

Validation:

 in-house validation

Standardisation:

 none

Related Methods:

Type:

 element-specific

Target DNA element:
CS-bar-STRHY

  • Oligonucleotides:

  • Forward Primer

  • Name:

    bar 2-5'

  • Sequence:

    actgggctccacgctctaca

  • Size:

    20

  • Reverse Primer

  • Name:

    bar 2-3'

  • Sequence:

    aaacccacgtcatgccagttc

  • Size:

    21

  • Probe

  • Name:

    Bar-1-Taq

  • Sequence:

    atgctgcgggcggccggcttcaagcacgg

  • Size:

    29

  • Amplicon:

  • Sequence:

    actgggctccacgctctacacccacctgctgaagtccctggaggcacagggcttcaagagcgtggtcgctgtcatcgggctgcccaacgacccgagcgtgcgcatgcacgaggcgctcggatatgccccccgcggcatgctgcgggcggccggcttcaagcacgggaactggcatgacgtgggttt

  • Size:

    186

Documents

Citation Type Local copy
Mano J, Shigemitsu N, Futo S, Akiyama H, Teshima R, Hino A, Furui S, Kitta K (2009) Real-Time PCR array as a universal platform for the detection of genetically modified crops and its application in identifying unapproved genetically modified crops in Japan, Journal of Agricultural and Food Chemistry, 57, 26-37Link to the document url. peer-reviewed article