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Detection method details
Name: |
QL-ELE-P-Rice actin-F/P-Rice actin-R |
Description: |
Qualitative real-time PCR (TaqMan) method for detection of rice actin 1 promoter (P-ractI) (Debode et al. 2013). |
Comment: |
Target is the actin 1 promoter from rice (Oryza sativa) |
Validation: |
in-house validation |
Standardisation: |
none |
Related Methods: |
Type: |
element-specific |
Target DNA element: |
P-act1-ORYSA
|
-
Oligonucleotides:
Forward Primer
Name:
P-Rice actin-FSequence:
TCGAGGTCATTCATATGCTTGAGSize:
23
Reverse Primer
Name:
P-Rice actin-RSequence:
TTTTAACTGATGTTTTCACTTTTGACCSize:
27
Probe
Name:
P-Rice actin-PSequence:
AGAGAGTCGGGATAGTCCAAAATAAAACAAAGGTASize:
35
-
Amplicon:
Sequence:
TCGAGGTCATTCATATGCTTGAGAAGAGAGTCGGGATAGTCCAAAATAAAACAAAGGTAAGATTACCTGGTCAAAAGTGAAAACATCAGTTAAAASize:
95