Detection method details
Name: |
QT-EVE-ZM-002 |
Description: |
Quantitative real-time PCR (TaqMan) method for detection of maize event 5307 (EURL GMFF GMOMETHODS database as of 2014). |
Comment: |
Target is the 3' integration border region (IBR) between the insert of maize event 5307 and the maize host genome. |
Validation: |
ring trial validation |
Standardisation: |
EU reference method |
Related Methods: |
Type: |
event-specific |
Target GMO name: |
5307 |
-
Oligonucleotides:
Forward Primer
Name:
5307i3' forward primerSequence:
CATGGCCGTATCCGCAATGTGSize:
21
Reverse Primer
Name:
5307i3' reverse primerSequence:
TGCACCCTTTGCCAGTGGSize:
18
Probe
Name:
5307i3'-s2 probeSequence:
ACCACAATATACCCTCTTCCCTGGGCCAGSize:
29
-
Amplicon:
Sequence:
catggccgtatccgcaatgtgnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnaccacaatataccctcttccctgggccagnnnnnnnccactggcaaagggtgcaSize:
107
Documents
Citation | Type | Local copy |
---|---|---|
EU-RL GMFF GMOMETHODS database; QT-EVE-ZM-002 | EU reference method protocol |