Detection method details
Name: |
QL-ELE-B'nase-F-n4/BNaseR-n3 |
Description: |
Qualitative real-time PCR (TaqMan) method for detection of the barnase gene (Scholtens et al. 2013). |
Comment: |
Target is the barnase gene from bacterium Bacillus amyloliquefaciens. |
Validation: |
in-house validation |
Standardisation: |
none |
Related Methods: |
Type: |
element-specific |
Target DNA element: |
CS-barnase
|
-
Oligonucleotides:
Forward Primer
Name:
B'nase-F-n4Sequence:
TGGTACCGGTTATTCAACACGSize:
21
Reverse Primer
Name:
BNaseR-n3Sequence:
GAGGGCTTGTGCTTCTGATTTTSize:
22
Probe
Name:
BNaseP-n3Sequence:
GTCTGAAGATAATCCGCAACCCSize:
22
-
Amplicon:
Sequence:
tggtaccggttattcaacacgtttgacggggttgcggattatcttcagacatatcataagctacctgataattacattacaaaatcagaagcacaagccctcSize:
102
Documents
Citation | Type | Local copy |
---|---|---|
Scholtens et al. (2012) |