Help icon

Detection Image Detection method details

Name:

 QL-ELE-AgroBS2 F/AgroBS2 R

Description:

 Qualitative real-time PCR (TaqMan) method for detection of the Agrobacterium tumefaciens Ti plasmids of the octopine type aka Agroborder II.

Part of the qualitative triplex real-time PCR (TaqMan) method for detection of Ti plasmid (AgroBorder) sequences and a sequence of the Cassava vein mosaic virus promoter pat construct (P-CsVMV-pat) for screening for components of genetically modified organisms in food (Official Collection of Methods according to § 64 LFGB, L 00.00-176)

Comment:

 Target are border sequences from Agrobacterium tumefaciens Ti plasmids of the octopine type, which occurs as a construct-flanking sequence in various genetically modified plants.

Validation:

 ring trial validation

Standardisation:

 national standard

Related Methods:

 QL-ELE-AgroBS1 F/AgroBS1 R

Type:

 element-specific

Target DNA element:
V-Flanking Ti-ocs-RHIRD

  • Oligonucleotides:

  • Forward Primer

  • Name:

    AgroBS2 F

  • Sequence:

    ATTTTGATAAAACGACAAATTACG

  • Size:

    24

  • Reverse Primer

  • Name:

    AgroBS2 R

  • Sequence:

    TCGAAATAAAGATTTCCGAATTAG

  • Size:

    24

  • Probe

  • Name:

    AgroBS2

  • Sequence:

    CCGTCGTATTTATAGGCGAAAGCAATA

  • Size:

    30

  • Amplicon:

  • Sequence:

    attttgataaaacgacaaattacgatccgtcgtatttataggcgaaagcaataaacaaattattctaattcggaaatctttatttcga

  • Size:

    88

Documents

Citation Type Local copy
Detection of flanking Ti plasmid (AgroBorder) sequences as well as a sequence of the Cassava vein mosaic virus promoter-pat construct (P-CsVMV-pat) for screening for components from genetically modified organisms (GMOs) in food by multiplex real-time PCR (construct- and element-specific method). BVL Official Method Collection L 00.00-176 Online Publication (2022)Link to the document url. other document