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Detection Image Detection method details

Name:

 QT-ELE-00-002

Description:

 Quantitative real-time PCR (TaqMan) method for detection of phosphinothricin N-acetyltransferase gene (Weighardt et al., 2004) (EURL GMFF GMOMETHODS database as of February 5, 2020)

Comment:

 Target is the phosphinothricin N-acetyltransferase (pat) gene from Streptomyces viridochromogenes

Validation:

 ring trial validation

Standardisation:

 unknown

Related Methods:

Type:

 element-specific

Target DNA element:
CS-pat-STRVR

  • Oligonucleotides:

  • Forward Primer

  • Name:

    KVM-5

  • Sequence:

    TTGAGGGTGTTGTGGCTGGTA

  • Size:

    21

  • Reverse Primer

  • Name:

    KVM-6

  • Sequence:

    TGTCCAATCGTAAGCGTTCCT

  • Size:

    21

  • Probe

  • Name:

    Pat1

  • Sequence:

    CTTCCAGGGCCCAGCGTAAGCA

  • Size:

    22

  • Amplicon:

  • Sequence:

    ttgagggtgttgtggctggtantgcttacgctgggccctggaagnnnaggaacgcttacgattggaca

  • Size:

    68

Documents

Citation Type Local copy
Weighardt F., Barbati C., Paoletti C., Querci M., Kay S., De Beuckeleer M., Van den Eede G.; Real-time polymerase chain reaction-based approach for quantification of the pat gene in the T25 Zea mays event J AOAC Int 87:1342-1355 (2004)Link to the document url. peer-reviewed article
EU-RL GMFF GMOMETHODS database; QT-ELE-00-002Link to the document url. EU reference method protocol