Detection method details
Name: |
QT-TAX-ST-010 |
Description: |
Quantitative real-time PCR (TaqMan) method for detection of potato UDP-glucose pyrophosphorylase gene (EU-RL GMFF GMOMETHODS database as of January 22, 2014) |
Comment: |
Target is the endogenous UDP-glucose pyrophosphorylase (UGPase) gene of Solanum tuberosum |
Validation: |
ring trial validation |
Standardisation: |
EU reference method |
Related Methods: |
Type: |
taxon-specific |
Target Species name: |
Solanum tuberosum |
-
Oligonucleotides:
Forward Primer
Name:
UGP-af7Sequence:
GGACATGTGAAGAGACGGAGCSize:
21
Reverse Primer
Name:
UGP-ar8Sequence:
CCTACCTCTACCCCTCCGCSize:
19
Probe
Name:
UGP-sf1Sequence:
CTACCACCATTACCTCGCACCTCCTCASize:
27
-
Amplicon:
Sequence:
ggacatgtgaagagacggagcnnnnnnnnnnnnnntgaggaggtgcgaggtaatggtggtagnnnnnnngcnggaggggtagaggtaggSize:
89
Documents
Citation | Type | Local copy |
---|---|---|
EU-RL GMFF GMOMETHODS database; QT-TAX-ST-010 | EU reference method protocol |