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Detection Image Detection method details

Name:

 QT-EVE-ZM-024

Description:

 Quantitative PCR method for detection of maize event MON87411 (EURL GMFF GMOMETHODS database, as of April 21, 2017)

Comment:

 Target is the 3' integration border region (IBR) between the insert of maize event MON87411 and the maize host genome.

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

 QT-TAX-ZM-002

Type:

 event-specific

Target GMO name:

 MON87411

  • Oligonucleotides:

  • Forward Primer

  • Name:

    87411 QF

  • Sequence:

    CTCTGTAACAGAAAACACCATCTAGAG

  • Size:

    27

  • Reverse Primer

  • Name:

    87411 QR

  • Sequence:

    ACAAAAGTGAACTAGTTCTAGGGTAGAT

  • Size:

    28

  • Probe

  • Name:

    87411 QP

  • Sequence:

    CCGCGTTTAAACTATCAGTGTTTAGAGAAT

  • Size:

    30

  • Amplicon:

  • Sequence:

    ctctgtaaca gaaaacacca tctagagnnn ccgcgtttaa actatcagtg tttagagaat nnnnnnnnnn nnnnnnnnnn natctaccct agaactagtt cacttttgt

  • Size:

    109

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-EVE-ZM-024Link to the document url. EU reference method protocol