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Detection Image Detection method details

Name:

 QT-EVE-GM-014

Description:

 Quantitative PCR method for detection of soybean event DAS81419 (EURL GMFF GMOMETHODS database as of May 28, 2015)

Comment:

 Target is the 5' integration border region (IBR) between the insert of soybean event DAS81419 and the soybean host genome

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

 QT-TAX-GM-002

Type:

 event-specific

Target GMO name:

 DAS81419

  • Oligonucleotides:

  • Forward Primer

  • Name:

    DAS81419-f2

  • Sequence:

    TCTAGCTATATTTAGCACTTGATATTCAT

  • Size:

    29

  • Reverse Primer

  • Name:

    DAS81419-r1

  • Sequence:

    GCTTCAAGATCCCAACTTGCG

  • Size:

    21

  • Probe

  • Name:

    DAS81419-p3

  • Sequence:

    ATCAACAGGCACCGATGCGCACCG

  • Size:

    24

  • Amplicon:

  • Sequence:

    tctagctatatttagcacttgatattcatnnnnnnnnnnnnnnnnnnnnnnnnnnncggtgcgcatcggtgcctgttgatnnnncgcaagttgggatcttgaagc

  • Size:

    105

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-EVE-GM-014Link to the document url. EU reference method protocol