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Detection Image Detection method details

Name:

 QT-EVE-GH-004

Description:

 Quantitative real-time PCR (TaqMan) method for detection of cotton event MON531 (EU-RL GMFF GMOMETHODS database as of January 13, 2014)

Comment:

 Target is the 5' integration border region (IBR) between the insert of cotton event MON531 and the cotton host genome

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

 QT-TAX-GH-015

Type:

 event-specific

Target GMO name:

 MON531

  • Oligonucleotides:

  • Forward Primer

  • Name:

    531-F

  • Sequence:

    AACCAATGCCACCCCACTGA

  • Size:

    20

  • Reverse Primer

  • Name:

    531-R

  • Sequence:

    TCCCATTCGAGTTTCTCACGT

  • Size:

    21

  • Probe

  • Name:

    531-P

  • Sequence:

    TTGTCCCTCCACTTCTTCTC

  • Size:

    20

  • Amplicon:

  • Sequence:

    aaccaatgccaccccactgannnnnnnnnnngagaagaagtggagggacaaacgtgagaaactcgaatggga

  • Size:

    72

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-EVE-GH-004Link to the document url. EU reference method protocol