Help icon

Detection Image Detection method details

Name:

 QT-EVE-BN-004

Description:

 Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event GT73 (EU-RL GMFF GMOMETHODS database as of October 01, 2014)

Comment:

 Target is the 3' integration border region (IBR) between the insert of oilseed rape event GT73 and the oilseed rape host genome

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

 QT-TAX-BN-012, QT-TAX-FatA primer1/FatA primer2

Type:

 event-specific

Target GMO name:

 GT73

  • Oligonucleotides:

  • Forward Primer

  • Name:

    RT73 primer 1

  • Sequence:

    CCATATTGACCATCATACTCATTGCT

  • Size:

    26

  • Reverse Primer

  • Name:

    RT73 primer 2

  • Sequence:

    GCTTATACGAAGGCAAGAAAAGGA

  • Size:

    24

  • Probe

  • Name:

    RT73 Probe

  • Sequence:

    TTCCCGGACATGAAGATCATCCTCCTT

  • Size:

    27

  • Amplicon:

  • Sequence:

    ccatattgaccatcatactcattgctnnnnnnnnnnnnnttcccggacatgaagatcatcctccttnnnnnnnnnnnnnnnnnntccttttcttgccttcgtataagc

  • Size:

    108

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-EVE-BN-004Link to the document url. EU reference method protocol