Detection method details
Name: |
QT-EVE-ZM-007 |
Description: |
Quantitative real-time PCR (TaqMan) method for detection of maize event GA21 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) |
Comment: |
Target is the 5' integration border region (IBR) between the insert of maize event GA21 and the maize host genome |
Validation: |
ring trial validation |
Standardisation: |
EU reference method |
Related Methods: |
QT-TAX-ZM-011 |
Type: |
event-specific |
Target GMO name: |
GA21 |
-
Oligonucleotides:
Forward Primer
Name:
GA21 primer FSequence:
CTTATCGTTATGCTATTTGCAACTTTAGASize:
29
Reverse Primer
Name:
GA21 primer RSequence:
TGGCTCGCGATCCTCCTSize:
17
Probe
Name:
GA21 Probe PRSequence:
CATATACTAACTCATATCTCTTTCTCAACAGCAGGTGGGTSize:
40
-
Amplicon:
Sequence:
cttatcgttatgctatttgcaactttagancatatactaactcatatctctttctcaacagcaggtgggtnnnnnnnnnnnnnnnnnnnnnnnnnaggaggatcgcgagccaSize:
112
Documents
Citation | Type | Local copy |
---|---|---|
EU-RL GMFF GMOMETHODS database; QT-EVE-ZM-007 | EU reference method protocol |