Detection method details
Name: |
QT-EVE-GM-008 |
Description: |
Quantitative real-time PCR (TaqMan) method for detection of soybean event DP305423 (EU-RL GMFF GMOMETHODS database as of January 23, 2014) |
Comment: |
Target is the 3' integration border region (IBR) between the insert of soybean event DP305423 and the soybean host genome |
Validation: |
ring trial validation |
Standardisation: |
EU reference method |
Related Methods: |
QT-TAX-GM-002 |
Type: |
event-specific |
Target GMO name: |
DP305423 |
-
Oligonucleotides:
Forward Primer
Name:
DP305-f1Sequence:
CGTGTTCTCTTTTTGGCTAGCSize:
21
Reverse Primer
Name:
DP305-r5Sequence:
GTGACCAATGAATACATAACACAAACTASize:
28
Probe
Name:
DP305-pSequence:
TGACACAAATGATTTTCATACAAAAGTCGAGASize:
32
-
Amplicon:
Sequence:
cgtgttctctttttggctagcnnnnnnnnnnntctcgacttttgtatgaaaatcatttgtgtcantagtttgtgttatgtattcattggtcacSize:
93
Documents
Citation | Type | Local copy |
---|---|---|
EU-RL GMFF GMOMETHODS database; QT-EVE-GM-008 | EU reference method protocol |