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Detection Image Detection method details

Name:

 QL-EVE-OS-KM2_for/KM1_rev

Description:

 Qualitative real-time PCR (TaqMan) method for detection of rice event KMD1

Comment:

 Target is the 3' integration border region (IBR) between the insert of rice event KMD1 and the rice host genome.

The forward primer targets the pBn19 sequence from the insert, the reverse primer targets the rice host genome and the probe target the transition sequence between pBn19 and the host genome.

Validation:

 in-house validation

Standardisation:

 none

Related Methods:

Type:

 event-specific

Target GMO name:

 KMD1

  • Oligonucleotides:

  • Forward Primer

  • Name:

    KM2_for

  • Sequence:

    TCCGCAATGTGTTATTAAGTTGTCTAA

  • Size:

    27

  • Reverse Primer

  • Name:

    KM1_rev

  • Sequence:

    CCGATATGCCTGCCCATCT

  • Size:

    19

  • Probe

  • Name:

    KM-p

  • Sequence:

    CGTCAATTTGTTTACACCACAATATATCCCG

  • Size:

    31

  • Amplicon:

  • Sequence:

    TCCGCAATGTGTTATTAAGTTGTCTAAGCGTCAATTTGTTTACACCACAATATATCCCGAGATGGGCAGGCATATCGG

  • Size:

    78

Documents

Citation Type Local copy
Babekova R, Funk T, Pecoraro S et al. (2009). Development of an event-specific Real-time PCR detection method for the transgenic Bt rice line KMD1. Eur Food Res Technol, 228, 707-716Link to the document url. peer-reviewed article Download PDF pdf