Detection method details
Name: |
QL-ELE-00-017 |
Description: |
Qualitative real-time PCR (SYBRGreen) method for detection of P-35S CaMV (Barbau-Piednoir et al., 2014) |
Comment: |
Target is the P-35S CaMV from Cauliflower mosaic virus |
Validation: |
ring trial validation |
Standardisation: |
unknown |
Related Methods: |
QL-TAX-BN-003, QL-TAX-GM-001, QL-TAX-ZM-002 |
Type: |
element-specific |
Target DNA element: |
P-Cauliflower mosaic virus
|
-
Oligonucleotides:
Forward Primer
Name:
35S_N3FwdSequence:
AAAGCAAGTGGATTGATGTGATASize:
23
Reverse Primer
Name:
35S_N3RevSequence:
GGGTCTTGCGAAGGATAGTGSize:
20
-
Amplicon:
Sequence:
aaagcaagtggattgatgtgatannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncactatccttcgcaagacccSize:
75
Documents
| Citation | Type | Local copy |
|---|---|---|