Detection method details
Name: |
QL-ELE-00-019 |
Description: |
Qualitative real-time PCR (SYBRGreen) method for detection of CS-CP4epsps-RHIRD (Barbau-Piednoir et al., 2014) |
Comment: |
Target is the CS-CP4epsps-RHIRD from Agrobacterium tumefaciens
Two reverse primers are described that are meant to be used in PCR together. Primer CP4 Synthetic R (TGAAGGACCGGTGGGAGAT) binds to the nucleotide sequence of CS-CP4epsps-RHIRD in soybean, maize and cotton GMO events whereas primer CP4 synth Rbis (TGAAGGACCTGTGGGAGAT) recognizes a nucleotide sequence of CS-CP4epsps-RHIRD in GM rapeseed and sugar beet (Barbau-Piednoir et al., 2012). For Bioinformatical reasons, a degenerate primer was introduced in the reverse primer sequence (K=G/T) and in the amplicon. |
Validation: |
ring trial validation |
Standardisation: |
unknown |
Related Methods: |
QL-TAX-BN-003, QL-TAX-GM-001, QL-TAX-ZM-002 |
Type: |
element-specific |
Target DNA element: |
CS-CP4epsps-RHIRD
|
-
Oligonucleotides:
Forward Primer
Name:
CP4 Synthetic FSequence:
GCATGCTTCACGGTGCAASize:
18
Reverse Primer
Name:
CP4 Synthetic R / CP4 synth RbisSequence:
TGAAGGACCKGTGGGAGATSize:
19
-
Amplicon:
Sequence:
gcatgcttcacggtgcaannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnatctcccacmggtccttcaSize:
108