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Detection Image Detection method details

Name:

 QL-ELE-Patf-n2/Patr-n2

Description:

 Qualitative real-time PCR (TaqMan) method for detection of the pat gene (Xu et al., 2006)

Comment:

 target is the phosphinothricin N-acetyltransferase (pat) gene from bacterium Streptomyces viridochromogenes

Validation:

 in-house validation

Standardisation:

 none

Related Methods:

Type:

 element-specific

Target DNA element:
CS-pat-STRVR

  • Oligonucleotides:

  • Forward Primer

  • Name:

    Patf-n2

  • Sequence:

    GACAGAGCCACAAACACCACAA

  • Size:

    22

  • Reverse Primer

  • Name:

    Patr-n2

  • Sequence:

    CAATCGTAAGCGTTCCTAGCCT

  • Size:

    22

  • Probe

  • Name:

    Patp-n2

  • Sequence:

    GCCACAACACCCTCAACCTCA

  • Size:

    21

  • Amplicon:

  • Sequence:

    GACAGAGCCACAAACACCACAAGAGTGGATTGATGATCTAGAGAGGTTGCAAGATAGATACCCTTGGTTGGTTGCTGAGGTTGAGGGTGTTGTGGCTGGTATTGCTTACGCTGGGCCCTGGAAGGCTAGGAACGCTTACGATTG

  • Size:

    144

Documents

Citation Type Local copy
Xu J, Miao H, Wu H, Huang W, R. Tang R, Qiu M, Wen J, Zhu S, Li Y (2006) Screening genetically modified organisms using multiplex-PCR coupled with oligonucleotide microarray. Biosensors and Bioelectronics 22, 71-77 doi: 10-1016/j.bios.2005.12.001.Link to the document url.