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Detection Image Detection method details

Name:

 QL-ELE-AgroBS1 F/AgroBS1 R

Description:

 Qualitative real-time PCR (TaqMan) method for detection of the Agrobacterium tumefaciens Ti plasmids of the nopaline type aka Agroborder I.

Part of the qualitative triplex real-time PCR (TaqMan) method for detection of Ti plasmid (AgroBorder) sequences and a sequence of the Cassava vein mosaic virus promoter pat construct (P-CsVMV-pat) for screening for components of genetically modified organisms in food (Official Collection of Methods according to § 64 LFGB, L 00.00-176)

Comment:

 Target are sequences from Agrobacterium tumefaciens Ti plasmids of the nopaline type, which occurs as promoter in genetic engineering constructs and more frequently as construct flanking sequence in various genetically modified plants.

Validation:

 ring trial validation

Standardisation:

 national standard

Related Methods:

Type:

 element-specific

Target DNA element:
V-Flanking Ti-nos-RHIRD

  • Oligonucleotides:

  • Forward Primer

  • Name:

    AgroBS1 F

  • Sequence:

    TGATCATGAGCGGAGAATTAAGGG

  • Size:

    24

  • Reverse Primer

  • Name:

    AgroBS1 R

  • Sequence:

    CCAAACGTAAAACGGCTTGTCC

  • Size:

    22

  • Probe

  • Name:

    AgroBS1

  • Sequence:

    CGTTATGACCCCCGCCGATGACGC

  • Size:

    24

  • Amplicon:

  • Sequence:

    tgatcatgagcggagaattaagggagtcacgttatgacccccgccgatgacgcgggacaagccgttttacgtttgg

  • Size:

    76

Documents

Citation Type Local copy
Detection of flanking Ti plasmid (AgroBorder) sequences as well as a sequence of the Cassava vein mosaic virus promoter-pat construct (P-CsVMV-pat) for screening for components from genetically modified organisms (GMOs) in food by multiplex real-time PCR (construct- and element-specific method). BVL Official Method Collection L 00.00-176 Online Publication (2022)Link to the document url. other document