Detection method details
Name: |
QT-EVE-GH-002 |
Description: |
Quantitative real-time PCR (TaqMan) method for detection of cotton event LLCotton25 (EU-RL GMFF GMOMETHODS database as of January 13, 2014) |
Comment: |
Target is the 5' integration border region (IBR) between the insert of cotton event LLCotton25 and the cotton host genome |
Validation: |
ring trial validation |
Standardisation: |
EU reference method |
Related Methods: |
QT-TAX-GH-018 |
Type: |
event-specific |
Target GMO name: |
LLCotton25 |
-
Oligonucleotides:
Forward Primer
Name:
KVM156Sequence:
CAAGGAACTATTCAACTGAGSize:
20
Reverse Primer
Name:
KVM155Sequence:
CAGATTTTTGTGGGATTGGAATTCSize:
24
Probe
Name:
TM018Sequence:
CTTAACAGTACTCGGCCGTCGACCGCSize:
26
-
Amplicon:
Sequence:
caaggaactattcaactgagcttaacagtactcggccgtcgaccgcnnnnnnnnngaattccaatcccacaaaaatctgSize:
79
Documents
Citation | Type | Local copy |
---|---|---|
Mazzara M., Grazioli E., Savini C., Van Den Eede G.; Event-Specific Method for the Quantification of Cotton Line LLCotton25" Using Real-time PCR - Validation Report and Protocol - Cotton Seeds Sampling and DNA Extraction" Online Publication (2007) | EU reference method protocol | |
EU-RL GMFF GMOMETHODS database; QT-EVE-GH-002 | EU reference method protocol |