Detection method details
Name: |
QL-EVE-EC-001 |
Description: |
Qualitative real-time PCR (TaqMan) method for the detection of event PT73 (TM) (E. coli K-12 event AG3139) (EU-RL GMFF GMOMETHODS database as of November 24, 2017) |
Comment: |
Target is the 5' integration border region (IBR) between the insert of event PT73 (TM) and the bacterial host genome |
Validation: |
ring trial validation |
Standardisation: |
EU reference method |
Related Methods: |
Type: |
event-specific |
Target GMO name: |
PT73 E. coli (TM) |
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Oligonucleotides:
Forward Primer
Name:
TMD-ForSequence:
AATACCGTTAAACGTAAATTCTTTTTCTTTSize:
30
Reverse Primer
Name:
TMD-RevSequence:
TCCTCCCGGTTTTTTTCGTASize:
20
Probe
Name:
TMD-probeSequence:
AGATCGAGTATTCATTCGGTGTATTGATTCACTTGASize:
36
-
Amplicon:
Sequence:
aataccgttaaacgtaaattctttttctttnnagatcgagtattcattcggtgtattgattcacttganntacgaaaaaaaccgggaggaSize:
90
Documents
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