Detection method details
Name: |
QT-EVE-BN-003 |
Description: |
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event Rf3 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) |
Comment: |
Target is the 3' integration border region (IBR) between the insert of oilseed rape event Rf3 and the oilseed rape host genome |
Validation: |
ring trial validation |
Standardisation: |
EU reference method |
Related Methods: |
QT-TAX-BN-012 |
Type: |
event-specific |
Target GMO name: |
Rf3 |
-
Oligonucleotides:
Forward Primer
Name:
DPA165Sequence:
CATAAAGGAAGATGGAGACTTGAGSize:
24
Reverse Primer
Name:
KVM084Sequence:
AGCATTTAGCATGTACCATCAGACASize:
25
Probe
Name:
TM010Sequence:
CGCACGCTTATCGACCATAAGCCCASize:
25
-
Amplicon:
Sequence:
cataaaggaagatggagacttgagnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntgggcttatggtcgataagcgtgcgnntgtctgatggtacatgctaaatgctSize:
139
Documents
Citation | Type | Local copy |
---|---|---|
EU-RL GMFF GMOMETHODS database; QT-EVE-BN-003 | EU reference method protocol | |
Savini C., Bogni A., Mazzara M., Van Den Eede G.; Event-specific Method for the Quantification of Oilseed Rape Line Rf3 Using Real-time PCR - Validation Report and Protocol - Seeds Sampling and DNA Extraction Online Publication (2007) | EU reference method protocol |