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Detection Image Detection method details

Name:

 QT-ELE-00-001

Description:

 Quantitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (Feinberg et al., 2005)

Comment:

 Target is the Cauliflower mosaic virus 35S promoter (CaMV P-35S)

Validation:

 ring trial validation

Standardisation:

 unknown

Related Methods:

Type:

 element-specific

Target DNA element:
P-Cauliflower mosaic virus

  • Oligonucleotides:

  • Forward Primer

  • Name:

    sF

  • Sequence:

    CGTCTTCAAAGCAAGTGGATTG

  • Size:

    22

  • Reverse Primer

  • Name:

    sR

  • Sequence:

    TCTTGCGAAGGATAGTGGGATT

  • Size:

    22

  • Probe

  • Name:

    35S core

  • Sequence:

    TCTCCACTGACGTAAGGGATGACGCA

  • Size:

    26

  • Amplicon:

  • Sequence:

    cgtcttcaaagcaagtggattgnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnaatcccactatccttcgcaaga

  • Size:

    79

Documents

Citation Type Local copy
Feinberg M., Fernandez S., Cassard S., Bertheau Y.; Quantitation of 35S promoter in maize DNA extracts from genetically modified organisms using real-time polymerase chain reaction, part 2: interlaboratory study J AOAC Int 88:558-573 (2005)Link to the document url. peer-reviewed article
EU-RL GMFF GMOMETHODS database; QT-ELE-00-001Link to the document url. EU reference method protocol