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Detection Image Detection method details

Name:

 QT-EVE-BN-002

Description:

 Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event Ms8 (EU-RL GMFF GMOMETHODS database as of October 01, 2014)

Comment:

 Target is the 3' integration border region (IBR) between the insert of oilseed rape event Ms8 and the oilseed rape host genome

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

 QT-TAX-BN-012

Type:

 event-specific

Target GMO name:

 Ms8

  • Oligonucleotides:

  • Forward Primer

  • Name:

    KVM085

  • Sequence:

    GTTAGAAAAAGTAAACAATTAATATAGCCGG

  • Size:

    31

  • Reverse Primer

  • Name:

    HCA048

  • Sequence:

    GGAGGGTGTTTTTGGTTATC

  • Size:

    20

  • Probe

  • Name:

    TM011

  • Sequence:

    AATATAATCGACGGATCCCCGGGAATTC

  • Size:

    28

  • Amplicon:

  • Sequence:

    gttagaaaaagtaaacaattaatatagccggnnnnnnnnnnnnnnnnnnnnaatataatcgacggatccccgggaattcnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnngataaccaaaaacaccctcc

  • Size:

    130

Documents

Citation Type Local copy
Mazzara M., Bogni A., Savini C., Van Den Eede G.; Event-specific Method for the Quantification of Oilseed Rape Line Ms8 Using Real-time PCR - Validation Report and Protocol- Seeds Sampling and DNA Extraction of Oilseed Rape Online Publication (2007)Link to the document url. EU reference method protocol
EU-RL GMFF GMOMETHODS database; QT-EVE-BN-002Link to the document url. EU reference method protocol