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Detection Image Detection method details

Name:

 QT-CON-00-002

Description:

 Quantitative real-time PCR (TaqMan) method for detection of soybean event GTS 40-3-2 (Hird et al., 2003).

Comment:

 Target is the junction region between the chloroplast transit peptide (CTP) sequence from Petunia hybrida epsps gene and the CP4 epsps gene from Agrobacterium tumefaciens

Validation:

 ring trial validation

Standardisation:

 national standard

Related Methods:

 QT-TAX-GM-004

Type:

 construct-specific

Target GMO names:
GTS 40-3-2

  • Oligonucleotides:

  • Forward Primer

  • Name:

    RR-F

  • Sequence:

    GGATTTCAGCATCAGTGGCTACA

  • Size:

    23

  • Reverse Primer

  • Name:

    RR-R

  • Sequence:

    CCGGAAAGGCCAGAGGAT

  • Size:

    18

  • Probe

  • Name:

    RR soya

  • Sequence:

    CCGGCTGCTTGCACCGTGAAG

  • Size:

    21

  • Amplicon:

  • Sequence:

    ggatttcagcatcagtggctacannnnnnnnncttcacggtgcaagcagccggnnnnnnnnnnnnnnnnnatcctctggcctttccgg

  • Size:

    88

Documents

Citation Type Local copy
Hird H., Powell J., Johnson M.L., Oehlschlager S.; Determination of percentage of RoundUp Ready soya in soya flour using real-time polymerase chain reaction: interlaboratory study J AOAC Int 86:66-71 (2003)Link to the document url. peer-reviewed article
PCR reactions set up and amplification conditions Online Publication (2010); QT-CON-00-002Link to the document url. EU reference method protocol
EU-RL GMFF GMOMETHODS database; QT-CON-00-002Link to the document url. EU reference method protocol