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Detection Image Detection method details

Name:

 QT-EVE-BN-008

Description:

 Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event Topas 19/2 (EU-RL GMFF GMOMETHODS database as of July 21, 2014)

Comment:

 Target is the 3' integration border region (IBR) between the insert of oilseed rape event Topas 19/2 and the oilseed rape host genome

Validation:

 in-house validation

Standardisation:

 EU reference method

Related Methods:

 QT-TAX-BN-012

Type:

 event-specific

Target GMO name:

 Topas 19/2

  • Oligonucleotides:

  • Forward Primer

  • Name:

    MDB685

  • Sequence:

    GTTGCGGTTCTGTCAGTTCC

  • Size:

    20

  • Reverse Primer

  • Name:

    KVM180

  • Sequence:

    CGACCGGCGCTGATATATGA

  • Size:

    20

  • Probe

  • Name:

    TM029

  • Sequence:

    TCCCGCGTCATCGGCGG

  • Size:

    17

  • Amplicon:

  • Sequence:

    gttgcggttctgtcagttccnnnnnnnnnnnnnnnnntcccgcgtcatcggcggnnnnnnnnnnnnnnnnnnnnntcatatatcagcgccggtcg

  • Size:

    95

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-EVE-BN-008Link to the document url. EU reference method protocol