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Detection Image Detection method details

Name:

 QL-EVE-375LibF/376LibR

Description:

 Qualitative real-time PCR (TaqMan) method for detection of oilseed rape event Liberator (pHoe6/Ac) (BVL G30.40-6).

Comment:

 Target is the integration border region (IBR) between the insert of oilseed rape event Liberator (pHoe6/Ac) and the rapeseed host genome.

Validation:

 ring trial validation

Standardisation:

 national standard

Related Methods:

 QT-TAX-BN-012

Type:

 event-specific

Target GMO name:

 Liberator

  • Oligonucleotides:

  • Forward Primer

  • Name:

    375LibF

  • Sequence:

    CCATATTGACCATCATACTCCCC

  • Size:

    23

  • Reverse Primer

  • Name:

    376LibR

  • Sequence:

    TGACTTTGAACAATAATTTCAGTAACATATA

  • Size:

    28

  • Probe

  • Name:

    377LibS

  • Sequence:

    CCTGATGTAAACAATAAAATT

  • Size:

    21

  • Amplicon:

  • Sequence:

    ccatattgaccatcatactccccccctgatgtaaacaataaaattggtataattggagaatttactatatgttactgaaattattgttcaaagtca

  • Size:

    96

Documents

Citation Type Local copy
Real-time PCR detection of genetically modified oilseed rape lines Falcon GS40/90 and Liberator pHoe6/Ac - event-specific methods. BVL Official Method Collection G 30.40-06 Online Publication (2013)Link to the document url. other document