Detection method details
Name: |
QL-CON-GH-tFreeze-F/GH-tFreeze-R |
Description: |
Qualitative real-time PCR (TaqMan) method for detection of the junction between the growth hormone gene and the antifreeze terminator present in AquAdvantage Salmon (Official Collection of Methods according to § 64 LFGB, L 00.00-182). |
Comment: |
Target is the junction region between the growth hormone gene (CS-GHc2) sequence from Oncorhynchus tshawytscha and the antifreeze terminator (T-AFP) from Zoarces americanus. |
Validation: |
ring trial validation |
Standardisation: |
national standard |
Related Methods: |
Type: |
construct-specific |
Target GMO names: |
AquAdvantage Salmon
|
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Oligonucleotides:
Forward Primer
Name:
GH-tFreeze-FSequence:
CTCCACAGGTTTTGACATGTTCASize:
23
Reverse Primer
Name:
GH-tFreeze-RSequence:
GCCAGCAAGAGCCCATCTCSize:
19
Probe
Name:
GH-tFreeze-PSequence:
TTCCTAATCTGTATCTGGGAAACCGAACCCTSize:
31
-
Amplicon:
Sequence:
ctccacaggttttgacatgttcannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnngagatgggctcttgctggcSize:
75
Documents
Citation | Type | Local copy |
---|---|---|
Protocol QL-CON-GH-tFreeze-F/GH-tFreeze-R, Debode et al. 2017 | PCR Protocol | Download PDF |
Debode F., Janssen E., Marien A. et al. (2018) Detection of transgenic atlantic and Coho Salmon by real-time PCR. Food Analytical Methods,11(9),2396–2406 | peer-reviewed article |