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Detection Image Detection method details

Name:

 QL-ELE-00-016

Description:

 Qualitative real-time PCR (TaqMan) method for detection of the element coding for synthetic cry1Ab/Ac genes (ISO/TS 21569-6).

Comment:

 Targets are synthetic cry1Ab, cry1Ac or cry1Ab/Ac fusion genes derived from Bacillus thuringiensis.

Validation:

 ring trial validation

Standardisation:

 ISO standard

Related Methods:

Type:

 element-specific

Target DNA element:
CS-cry1Ab_1Ac_tr-SYNTH, CS-cry1Ac-BACTU, CS-cry1Ab-BACTU

  • Oligonucleotides:

  • Forward Primer

  • Name:

    Bt-F1(mod)

  • Sequence:

    GAGGAAATGCGTATTCAATTCAAC

  • Size:

    24

  • Reverse Primer

  • Name:

    Bt-R

  • Sequence:

    TTCTGGACTGCGAACAATGG

  • Size:

    20

  • Probe

  • Name:

    Bt-P

  • Sequence:

    ACATGAACAGCGCCTTGACCACAGC

  • Size:

    25

  • Amplicon:

  • Sequence:

    gaggaaatgcgtattcaattcaacgacatgaacagcgccttgaccacagctatcccattgttcgcagtccagaa

  • Size:

    74

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