Detection method details
Name: |
QT-EVE-ZM-003 |
Description: |
Quantitative PCR method for detection of maize event MON87427 (EU-RL GMFF GMOMETHODS database as of April 21, 2017). |
Comment: |
Target is the 5' integration border region (IBR) between the insert of maize event MON87427 and the maize host genome. |
Validation: |
ring trial validation |
Standardisation: |
EU reference method |
Related Methods: |
QT-TAX-ZM-002 |
Type: |
event-specific |
Target GMO name: |
MON87427 |
-
Oligonucleotides:
Forward Primer
Name:
MON87427 primer1Sequence:
ACGGAAACGGTCGGGTCAAATGSize:
22
Reverse Primer
Name:
MON87427 primer 2Sequence:
CCATGTAGATTTCCCGGTTTTCTCSize:
24
Probe
Name:
MON87427 probeSequence:
TCGGGACAATATGGAGAAAAAGAAAGAGSize:
28
-
Amplicon:
Sequence:
acggaaacgg tcgggtcaaa tgnnnnnnnt cgggacaata tggagaaaaa gaaagagnnn nnnnnnnnnn ngagaaaacc gggaaatcta catggSize:
95
Documents
Citation | Type | Local copy |
---|---|---|
EU-RL GMFF GMOMETHODS database; QT-EVE-ZM-003 | EU reference method protocol |