Detection method details
Name: |
QL-EVE-EC-002 |
Description: |
Qualitative real-time PCR (TaqMan) method for the detection of event PL73 (LM) (E. coli K-12 strain 19E) (EURL GMFF GMOMETHODS database as of November 24, 2017) |
Comment: |
Target is the integration site of event PL73 (LM) corresponding to the 5’ flanking region of bacterial origin and the integration sequence inserted into the bacterial genome (insert to E. coli junction) |
Validation: |
ring trial validation |
Standardisation: |
EU reference method |
Related Methods: |
Type: |
event-specific |
Target GMO name: |
PL73 E. coli (LM) |
-
Oligonucleotides:
Forward Primer
Name:
LMA forSequence:
GGTTATCCAGTAATAGCCATCTTCATCSize:
27
Reverse Primer
Name:
LMA revSequence:
CCTCCCGGTTTTTTTCGTACTTSize:
22
Probe
Name:
LMA probeSequence:
CCGTCGCCGCTGTATTGATTCACTTGSize:
26
-
Amplicon:
Sequence:
ggttatccagtaatagccatcttcatcnnnnnnnnnnccgtcgccgctgtattgattcacttgaagtacgaaaaaaaccgggaggSize:
85
Documents
Citation | Type | Local copy |
---|---|---|
EU-RL GMFF GMOMETHODS database; QL-EVE-EC-002 | EU reference method protocol |