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Detection Image Detection method details

Name:

 QL-CON-CsVMV F/patsyn R

Description:

 Qualitative real-time PCR (TaqMan) method for detection of the junction between the Cassava vein mosaic virus promoter (P-CsVMV) and the phosphinothricin N-acetyltransferase (pat) gene.

Part of the qualitative triplex real-time PCR (TaqMan) method for detection of Ti plasmid (AgroBorder) sequences and a sequence of the Cassava vein mosaic virus promoter pat construct (P-CsVMV-pat) for screening for components of genetically modified organisms in food (Official Collection of Methods according to § 64 LFGB, L 00.00-176)

Comment:

 Target is the junction region between the Cassava vein mosaic virus Promoter (P-CsVMV) and the phosphinothricin N-acetyltransferase gene (pat) from Streptomyces viridochromogenes.

Validation:

 ring trial validation

Standardisation:

 national standard

Related Methods:

 QL-ELE-AgroBS1 F/AgroBS1 R, QL-ELE-AgroBS2 F/AgroBS2 R

Type:

 construct-specific

Target GMO names:
DAS44406, DAS68416, DAS81419

  • Oligonucleotides:

  • Forward Primer

  • Name:

    CsVMV F

  • Sequence:

    AGTACTGAGGATACAACTTCAGAGA

  • Size:

    25

  • Reverse Primer

  • Name:

    patsyn R

  • Sequence:

    GCGGCCATATCAGCTGCTGT

  • Size:

    20

  • Probe

  • Name:

    patsynp

  • Sequence:

    CCGGAGAGGAGACCAGTTGAGATTAG

  • Size:

    26

  • Amplicon:

  • Sequence:

    AGTACTGAGGATACAACTTCAGAGAnnnnnnnnnnnnnnnnnnnnnnnnnnnnnCCGGAGAGGAGACCAGTTGAGATTAGnnnnnnnACAGCAGCTGATATGGCCGC

  • Size:

    107

Documents

Citation Type Local copy
Detection of flanking Ti plasmid (AgroBorder) sequences as well as a sequence of the Cassava vein mosaic virus promoter-pat construct (P-CsVMV-pat) for screening for components from genetically modified organisms (GMOs) in food by multiplex real-time PCR (construct- and element-specific method). BVL Official Method Collection L 00.00-176 Online Publication (2022)Link to the document url. other document