Detection method details
Name: |
QT-TAX-BN-002 |
Description: |
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape cruciferin storage protein (EU-RL GMFF GMOMETHODS database as of January 27, 2014).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 4 |
Comment: |
Target is the endogenous cruciferin storage protein (BnC1) gene from Brassica napus.
This assay is NOT recommended for GMO quantitative analysis because of a secondary target with mismatches in the primers and probe annealing region. |
Validation: |
ring trial validation |
Standardisation: |
EU reference method |
Related Methods: |
Type: |
taxon-specific |
Target Species name: |
Brassica napus |
-
Oligonucleotides:
Forward Primer
Name:
ccf RSequence:
GCTTCCGTGATATGCACCAGAAAGSize:
24
Reverse Primer
Name:
ccf FSequence:
ATTGGGCTACACCGGGATGTGTSize:
22
Probe
Name:
ccf PSequence:
CGATGGTGTCCCCAGTCCTTATGTGCTCSize:
28
-
Amplicon:
Sequence:
gcttccgtgatatgcaccagaaagnngagcacataaggactggggacaccatcgnnacacatcccggtgtagcccaatSize:
78
Documents
Citation | Type | Local copy |
---|---|---|
Jacchia S., Kagkli D.F., Lievens A., Angers-Loustau A., Savini C., Emons H., Mazzara M. (2018) Identification of single target taxon-specific reference assays for the most commonly genetically transformed crops using digital droplet PCR. Food Control 93,191-200 | peer-reviewed article | |
EU-RL GMFF GMOMETHODS database; QT-TAX-BN-002 | EU reference method protocol |