Detection method details
Name: |
QL-TAX-BN-004 |
Description: |
Qualitative conventional PCR method for detection of oilseed rape high mobility group protein I/Y gene (Pan et al., 2007). |
Comment: |
Target is the high mobility group protein I/Y (HMG IY) gene from rapeseed (Brassica napus) |
Validation: |
ring trial validation |
Standardisation: |
unknown |
Related Methods: |
Type: |
taxon-specific |
Target Species name: |
Brassica napus |
-
Oligonucleotides:
Forward Primer
Name:
HMG-1Sequence:
GGTCGTCCTCCTAAGGCGAAAGSize:
22
Reverse Primer
Name:
HMG-2Sequence:
GCAACCAACAGGCACCATCSize:
19
-
Amplicon:
Sequence:
ggtcgtcctcctaaggcgaaagnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnngatggtgcctgttggttgcSize:
219
Documents
Citation | Type | Local copy |
---|---|---|
EU-RL GMFF GMOMETHODS database; QL-TAX-BN-004 | EU reference method protocol | |
Pan L., Zhang S., Yang L., Broll H., Tian F., Zhang D.; Interlaboratory trial validation of an event-specific qualitative polymerase chain reaction-based detection method for genetically modified RT73 rapeseed J AOAC Int 90:1639-1646 (2007) | peer-reviewed article |