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Detection Image Detection method details

Name:

 QL-CON-00-003

Description:

 Qualitative conventional PCR method for detection of maize event Bt11 (ISO 21569)

Comment:

 target is the junction region between the Intron 2 (IVS2) from the maize alcohol dehydrogenase 1 (adh1) gene and the phosphinothricin N-acetyltransferase (pat) gene

Validation:

 ring trial validation

Standardisation:

 ISO standard

Related Methods:

Type:

 construct-specific

Target GMO names:
Bt10 maize, Bt11

  • Oligonucleotides:

  • Forward Primer

  • Name:

    IVS2-2

  • Sequence:

    CTGGGAGGCCAAGGTATCTAAT

  • Size:

    22

  • Reverse Primer

  • Name:

    PAT-B

  • Sequence:

    GCTGCTGTAGCTGGCCTAATCT

  • Size:

    22

  • Amplicon:

  • Sequence:

    ctgggaggccaaggtatctaatnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnagattaggccagctacagcagc

  • Size:

    189

Documents

Citation Type Local copy
Foodstuffs - Methods of analysis for the detection of genetically modified organisms and derived products - Qualitative nucleic acid based methods ISO 21569:1-69 (2005)Link to the document url. other document
EU-RL GMFF GMOMETHODS database; QL-CON-00-003Link to the document url. EU reference method protocol