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Detection Image Detection method details

Name:

 QT-EVE-BN-014

Description:

 Quantitative real-time PCR (TaqMan) for detection of oilseed rape event MON94100 (EU-RL GMFF GMOMETHODS database as of October 12, 2022)

Comment:

 Target is the 5' integration border region (IBR) between the insert of oilseed rape event MON94100 and the oilseed rape host genome.

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

 QT-TAX-BN-001

Type:

 event-specific

Target GMO name:

 MON94100

  • Oligonucleotides:

  • Forward Primer

  • Name:

    MON 94100 primer 1

  • Sequence:

    CACCATCTAATGAATAGTCACCAAAATAACG

  • Size:

    31

  • Reverse Primer

  • Name:

    MON 94100 primer 2

  • Sequence:

    CTATTCGGGCCTAACTTTTGGTGTG

  • Size:

    25

  • Probe

  • Name:

    MON 94100 probe

  • Sequence:

    TGATGCTGACTGGTGTCAA

  • Size:

    19

  • Amplicon:

  • Sequence:

    caccatctaa tgaatagtca ccaaaataac gnnnnnnnnn nnnnttgaca ccagtcagca tcancacacc aaaagttagg cccgaatag

  • Size:

    89

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-EVE-BN-014Link to the document url. EU reference method protocol