Detection method details
Name: |
QT-ELE-NOS ter 2-5' /NOS ter 2-3' |
Description: |
Quantitative real-time PCR (TaqMan) method for detection of T-nos (Kodama et al., 2009) |
Comment: |
target is the nopaline synthase terminator (T-nos) from bacterium Agrobacterium tumefaciens. Amplicon was generated from cotton LLcotton25 (GenBank HQ233646.1) |
Validation: |
ring trial validation |
Standardisation: |
none |
Related Methods: |
Type: |
element-specific |
Target DNA element: |
T-nos-RHIRD
|
-
Oligonucleotides:
Forward Primer
Name:
NOS ter 2-5'Sequence:
GTCTTGCGATGATTATCATATAATTTCTGSize:
29
Reverse Primer
Name:
NOS ter 2-3'Sequence:
CGCTATATTTTGTTTTCTATCGCGTSize:
25
Probe
Name:
NOS-TaqSequence:
AGATGGGTTTTTATGATTAGAGTCCCGCAASize:
30
-
Amplicon:
Sequence:
GTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATGTAATGCATGACGTTATTTATGAGATGGGTTTTTATGATTAGAGTCCCGCAATTATACATTTAATACGCGATAGAAAACAAAATATAGCGSize:
151