Detection method details
Name: |
QT-ELE-00-003 |
Description: |
Quantitative real-time PCR (TaqMan) method for detection of synthetic cryIA(b) gene (ISO/FDIS 21570) |
Comment: |
Target is the synthetic cryIA(b) gene |
Validation: |
ring trial validation |
Standardisation: |
ISO standard |
Related Methods: |
Type: |
element-specific |
Target DNA element: |
CS-cry1Ab-BACTU
|
-
Oligonucleotides:
Forward Primer
Name:
CRY2-FSequence:
CCCATCGACATCAGCCTGAGCSize:
21
Reverse Primer
Name:
CRY2-RSequence:
CAGGAAGGCGTCCCACTGGCSize:
20
Probe
Name:
BTSYNSequence:
ATGTCCACCAGGCCCAGCACGSize:
21
-
Amplicon:
Sequence:
cccatcgacatcagcctgagcnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnngccagtgggacgccttcctgSize:
129