Help icon

Detection Image Detection method details

Name:

 QL-ELE-00-014

Description:

 Qualitative real-time PCR (TaqMan) method for detection of the element coding for phosphinothricin N-acetyltransferase (CS-bar-STRHY) gene from Streptomyces hygroscopicus (ISO 21569 AMD1)

Comment:

 Target is the phosphinothricin N-acetyltransferase (CS-bar-STRHY) gene from the bacterium Streptomyces hygroscopicus

Validation:

 ring trial validation

Standardisation:

 ISO standard

Related Methods:

Type:

 element-specific

Target DNA element:
CS-bar-STRHY

  • Oligonucleotides:

  • Forward Primer

  • Name:

    RapB-F1

  • Sequence:

    acaagcacggtcaacttcc

  • Size:

    19

  • Reverse Primer

  • Name:

    RapB-R1

  • Sequence:

    gaggtcgtccgtccactc

  • Size:

    18

  • Probe

  • Name:

    RapB-S1

  • Sequence:

    taccgagccgcaggaacc

  • Size:

    18

  • Amplicon:

  • Sequence:

    acaagcacggtcaacttccgtaccgagccgcaggaaccgcaggagtggacggacgacctc

  • Size:

    60

Documents

Citation Type Local copy
Grohmann L., Brunen-Nieweler C., Nemeth A., Waiblinger H.U.; Collaborative trial validation studies of real-time PCR-based GMO screening methods for detection of the bar gene and the ctp2-cp4epsps construct J. Agric. Food Chem. 57:8913-8920 (2009)Link to the document url. peer-reviewed article
EU-RL GMFF GMOMETHODS database; QL-ELE-00-014Link to the document url. EU reference method protocol